Basic Stereology for Biologists and NeuroscientistsHow does one determine the size of an organelle? The length of a mass of capillaries? The number of synapses in the brain?

Stereological techniques can be used to estimate the number, length, surface area, and volume of structures in biological cells and tissues. Measurements are made on two-dimensional images or sections of a structure, and then mathematical rules are applied to generate a meaningful description of its three-dimensional geometry. Our newest book, Basic Stereology for Biologists and Neuroscientists, provides a practical guide to designing and critically evaluating stereological studies of the nervous system and other tissues.

“Over the past two decades, a large number of scientific papers have been published that collectively represent a paradigm shift in thinking about how to derive meaningful quantitative data about structural features in biological tissues,” writes the author, Mark West. “These are the design-based, unbiased stereological methods.”

These new stereological methods, the focus of the book, are introduced in “Introduction to Stereology,” a freely accessible article from Cold Spring Harbor Protocols. The book will be essential reading for neurobiologists and cell biologists interested in generating accurate representations of cell and tissue architecture.  For more information, click here.