Immunoimaging is rapidly developing from a merely descriptive technique into a set of methods and analytical tools that can be used to quantify and characterize an immune response at the cellular level. In the February issue of Cold Spring Harbor Protocols, Ian Parker and colleagues from the University of California, Irvine present Immunoimaging: Studying Immune System Dynamics Using Two-Photon Microscopy. The article outlines the hardware required for immunoimaging and discusses methods for quantitative analysis of multidimensional image stacks. As one of our featured articles for February, this overview is freely available to subscribers and nonsubscribers alike.

The issue also contains protocols from the same authors for a General Approach to Adoptive Transfer and Cell Labeling for Immunoimaging, Induction of an Immune Response for Imaging Antigen-Presenting Cell/T-Cell Interactions, In Situ Lymph Node Imaging and In Vivo Lymph Node Imaging.