The Drosophila neuromuscular junction (NMJ) provides a superb model system for investigating the cellular and molecular mechanisms of synaptic transmission. The NMJ is large, easily accessed and its genetics are well-characterized. It shares many structural and functional similarities to synapses in other animals, including humans. In the September issue of Cold Spring Harbor Protocols, Bing Zhang and Bryan Stewart present an essential set of primers for electrophysiological recording from the Drosophila NMJ. The issue contains a detailed explanation of the Equipment Setup necessary, as well as instructions for Fabrication of Microelectrodes, Suction Electrodes, and Focal Electrodes. Protocols for Electrophysiological Recording from a ‘Model’ Cell, Electrophysiological Recording from Drosophila Larval Body-Wall Muscles, Voltage-Clamp Analysis of Synaptic Transmission at the Drosophila Larval Neuromuscular Junction, and Focal Recording of Synaptic Currents from Single Boutons at the Drosophila Neuromuscular Junction are also included. These protocols are adapted from Drosophila Neurobiology: A Laboratory Manual. Based on Cold Spring Harbor Laboratory’s long-running course, this manual has rapidly become an important resource for any neuroscience lab.