The rapid pace of technological progress in biological imaging has provided great insight into the processes of embryonic development. But for higher organisms with opaque eggs or internal development, optical access to the embryo is limited. While various embryonic culture methods are available, vertebrate development is best studied in an intact embryo model, one in which the natural environment has not been disrupted. In the June issue of Cold Spring Harbor Protocols, Paul Kulesa and colleagues from the Stowers Institute for Medical Research present In Ovo Live Imaging of Avian Embryos, a detailed set of instructions for time-lapse imaging of fluorescently labeled cells within a living avian embryo. During the procedure, a hole is made in the shell, and a Teflon membrane that is oxygen-permeable and liquid-impermeable is used to provide a window for visualization of the embryo via confocal or two-photon microscopy. Imaging can take place for up to five days without dehydration or degradation of the normal developmental environment. As one of June’s featured articles, the protocol is freely available to subscribers and nonsubscribers alike. Kulesa’s group also supplies a second protocol in the issue, covering Multi-Position Photoactivation and Multi-Time Acquisition for Large-Scale Cell Tracing in Avian Embryos, a technique that produced June’s cover image.