One of the reasons Cold Spring Harbor Protocols exists is to allow us to keep valuable information available after the books where it was published go out of print. One of April’s featured articles, Optimization and Troubleshooting in PCR, is a great example of this.

Our PCR Primer manual has gone out of print, but the contents are still relevant and important to researchers in many labs. PCR is often difficult to optimize, and failure to do so can lead to undefined and unwanted products, or a complete lack of amplification altogether. To help avoid these issues, Kenneth Roux from Florida State University wrote the chapter that has been adapted for publication here. The article addresses various optimization strategies including touchdown PCR and hot-start PCR. Magnesium concentration, buffer pH, and cycling conditions are also considered. Like all our featured articles, Optimization and Troubleshooting in PCR is freely accessible to subscribers and non-subscribers alike.

This joins our collection of other valuable articles from PCR Primer like PCR Primer Design, Strategies for Overcoming PCR Inhibition, and Setting Up a PCR Laboratory. A complete listing of our PCR-related articles can be found here.