February’s issue of CSH Protocols has just posted, and one of our featured articles this month is The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro, from Juan Carlos Zúñiga-Pflücker’s laboratory at the University of Toronto. By using OP9-DL1 cells as a support system, researchers can study the differentiation of embryonic stem cells into mature components of the immune system. The article contains a series of protocols describing the establishment, maintenance, and storage of OP9 and OP9-DL1 cells; the co-culture of these cells with embryonic stem cells or hematopoietic stem cells from fetal liver or bone marrow; and the in vitro differentiation of the stem cells into lymphocytes. The OP9-DL1 system has been useful in addressing questions about the cellular and molecular regulation of T-lymphocyte lineage commitment, pre-T cell receptor signaling (Beta-selection), functional characteristics of progenitor T cells, and maturation of functional CD8 T cells.

Like all of our featured articles, this set of protocols is freely available to subscribers and non-subscribers.