As part of our mission to publish as many “gold standard” laboratory techniques as possible, I’m happy this month to include set of protocols covering the use of bromodeoxyuridine (BrdU) incorporation as a means of measuring DNA replication. The number of cells going through the cell cycle and their rate of progression are important indicators of cell growth. BrdU is a thymidine analog, which gets incorporated into new strands of DNA in a replicating cell in place of thymidine. It can then be detected with anti-BrdU antibodies. August’s issue of CSH Protocols provides three protocols for this method, from Dean Jackson and Peter R. Cook:
Analyzing DNA Replication I: Labeling Animals, Tissues, and Cells with Bromodeoxyuridine (BrdU)
Analyzing DNA Replication II: Fixation and Processing of Tissues and Cells Labeled with Bromodeoxyuridine (BrdU)
Analyzing DNA Replication III: Antibody Labeling of Incorporated Bromodeoxyuridine (BrdU) in Tissues and Cells