July’s issue of CSH Protocols includes two highly valuable compendiums of recipes and methods for staining cells, derived from Scion Publishing’s book, Histological and Histochemical Methods (4th Edition). The book is an information-dense treasure trove for cell biologists and microscopists, with over 600 pages of detailed instructions for sample preparation and processing, immunohistochemistry, and the staining of various cell types, tissues, structures and molecules. Nuclear Stains, by John Alan Kiernan covers methods for cationic, anionic, and metal complexing dyes. Anionic Counterstains (also by Kiernan) gives a set of methods for counterstaining with eosin, fast green and metanil yellow. The good folks at Scion have made these protocols freely accessible for both subscribers and non-subscribers, so be sure to take a look.


Histological and Histochemical Methods