Immunohistochemistry (the localization of proteins in a tissue by binding antibodies to specific antigens) is a technique where one protocol definitely does not fit all. Each model organism seems to have its own quirks, whether it be in the fixative used, the methods needed for antibody penetration, issues with autofluorescence or even just figuring out which cross-species antibodies work in a given system. To that end, we’ve been working on expanding our coverage of immunohistological protocols. The February issue of CSH Protocols brings methods for plant sections, using both avidin-biotin and alk-phos, as well as a method for whole-mount immunocytochemistry in Xenopus embryos from John Wallingford’s lab at the University of Texas (they provided the lovely cover image for this month).

—article continues—

These join our already published protocols for immunohistochemistry in Mouse (sections and whole-mount), Drosophila (imaginal discs [fluorescence], imaginal discs [avidin-biotin-HRP], S2 cells, tissues, embryos [colorimetric], embryos [fluorescence]), Zebrafish eyes (expect more zebrafish protocols shortly), Yeast, C. elegans, and general tissue sections.