Our protocol on Preparation of GST Fusion Proteins has seen a great deal of activity on the site, so this month we’ve supplemented it with several other protocols and background articles. This should give a better set of tools to do GST pull-downs and Far Westerns.

The articles are as follows:
Identification of Protein-Protein Interactions with Glutathione-S-Transferase (GST) Fusion Proteins

GST Pull-down

Far Western: Labeling GST Fusion Proteins

Far Western: Probing Membranes