May 2007


CSH Protocols is meant to be a dynamic set of information. If it’s just a static collection of articles that never changes, then it’s no better than the paper manuals that came before. Just reproducing books online seems a waste of the potential that online publishing offers. So expect things to continuously evolve… (more…)

With the rapid growth of siRNA techniques in so many experimental systems, it’s important to know your options for getting those RNAs into your cells or organism of choice. This month CSH Protocols presents four different methods for delivering siRNAs and shRNAs into various organisms. (more…)

A quick spin around the web reveals all sorts of interesting websites for biology, and all sorts of highly used, interactive forums and social networks. However, there doesn’t seem to be any crossover between the two. Why is this? (more…)

May’s Issue of CSH Protocols features a set of methods for Fluorescent In Situ Hybridization (FISH) from a group of scientists at Ludwig-Maximilians University Munich. While In Situ Hybridization dates back to 1969 (when several groups independently worked out methods), non-radioactive means of labeling probes didn’t come to the forefront until the 1980s. (more…)

CSH Protocols’ May Issue features a set of basic techniques for working with Xenopus laevis, the African Clawed Frog. While amphibians were used for experimental embryology as far back as the 1880′s, Xenopus became the amphibian of choice in the 1950′s, greatly aided by the work of John Gurdon… (more…)

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